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Briefly, adult fat bodies were dissected in 1 phosphate buffered saline (PBS), fixed 20 min in 4% paraformaldehyde at room temperature (RT), rinsed 5 in 1 PBS, then permeabilized for 30 min at RT in 1xPBS + 0.3% Triton X-100

Garrino L, Picco E, Finiguerra I, Rossi D, Simone P, Roccatello D

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It is also worth stating that even within the Semax literature the doseresponse relationship is not cleanly established for cognition in healthy subjects the human ACTH(4-10) attention work is old, small, and produced inconsistent, sometimes negative results